Coding
Part:BBa_K2984037:Design
Designed by: Darius Rauch Group: iGEM19_Humboldt_Berlin (2019-10-16)
L1c-Psad-PtxD-Rbcs2
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 4
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1790
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Selection marker for C.reinhardtii designed for Transformation in Clamydomnasd and further cloning into Level 2 backbone with MoClo Standard
Source
The construct consist of the four three Level 0 parts Psad (BBa_K2984008), ptxD(BBa_K2984026), and Rbcs2 (BBa_K2984018) which were cloned into the level 1 Backbone (BBa_K2984002) using the MoClo Standard.